Principle of Operation
High performance capillary electrophoresis is based on the differential migration of components of aqueous samples within a narrow fused silica capillary driven by an electric field. Separated solutes are quantitatively detected at the capillary outlet by a high sensitive optical system based on direct or indirect UV absorbance.
Aeas of Application
Environmental analysis: water and soil quality monitoring (cations, anions, herbicides, pesticides);
Food industry: analysis of beverages and juices (cations, anions, vitamins, organic acids, preservatives, antioxidants, dyes, sweeteners); mineral water (cations, anions); wines and brandies (cations, organic acids, polyphenols, aldehydes, sulfite); beer (inorganic cations and anions, hop and bitter acids, sulfite, amino acids, organic acids, vitamins); milk (proteins, antibiotics); tea, coffee (caffeine, polyphenols); foodstuff (amino acids, synthetic dyes, organic acids, amines, proteins, melamine);
Agriculture: grain and grain products (amino acids), mixed fodders (cations, amino acids, amines, vitamins);
Chemical industry: technological monitoring, analysis of raw material;
Pharmaceutical industry: patent medicines analysis, technological monitoring, and enantiomers separation;
Biochemistry: analysis of proteins by capillary isoelectric focusing technique, pharmacokinetics studies;
Forensic studies: analysis of explosives and trace detection, drugs analysis, analysis of writing paper components.
Analycal Characteristics
Detectors
- CAPEL-205 is equipped with a spectrophotometric detector with a wavelength range of 190 - 400 nm.
Light source
Vials
- Standard centrifuge-type (e.g.Eppendorf-type)1.5 ml, minimal sample volume 100 µl.
Injection modes
- Sample injections by pressure, vacuum or electrokinetically.
- Controlled pressure profile of injection with pressure values from – 100 mbar to + 100 mbar.
- Electrokinetic injection at (+/-) 1 – 30 kV considering time for voltage ramping.
- Programmable injection time / pressure / voltage.
Analysis
- With a Voltage from -30 kV to +30 kV with a 1 kV step and an electronic polarity switch.
- Current from 0 to 300 µA.
Elforun software features
- bination of instrument controlling, data acquisition and data processing;
- Real time electropherogram visualization;
- Electropherogram data processing with overlay mode and basic mathematical operations;
- Computation of electrophoresis system parameters;
- Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors;
- Extensive data processing algorithm customization;
- GLP and GALP compliance.
Equipment and Options
Capillary electrophoresis system CAPEL-205;
Spare capillary cassettes;
Elforun software package;
Kits for analyses (by request); most of CE-kits of any manufacturers are compatible with Capel-205.
Technical Spécifications
Detector :
- Spectrophotometer 190-400 nm
Analysis :
- Constant voltage, from –30 kV to +30 kV in 1 kV steps
- Automatic (electronic) polarity switch
- Current 0 - 300 mkA
- Pressure up to 100 mbar
- Programmable changing of wavelength, pressure and voltage during analysis
Injection :
- By voltage, from –30 kV to +30 kV in 1 kV steps;
- By pressure, from –100 to 100 mbar in 1 mbar steps
Sampler :
- Autosamplers for 59 vials (standard Eppendorf-type 1.5 mL)
Rinsing :
- By pressure, 500–2000 mbar in 1 mbar steps
Capillary :
- Length: 42–120 cm
- Internal diameter: 50, 75 μm
Capillary thermostating :
- Liquid thermostating, from -10 up to+ 30 °C from ambient temperature, ±0.1°C
Controlled parameters :
- Wavelength
- Pressure
- Temperature
- Voltage
Power requirements :
Power consumption :
Size :
Weight :
