Principle of Operation
High performance capillary electrophoresis is based on the differential migration of components of aqueous samples within a narrow fused silica capillary driven by an electric field. Separated solutes are quantitatively detected at the capillary outlet by a high sensitive optical system based on direct or indirect UV absorbance.
Areas of Application
Environmental analysis: water and soil quality monitoring (cations, anions, herbicides, pesticides);
Food industry: analysis of beverages and juices (cations, anions, vitamins, organic acids, preservatives, antioxidants, dyes, sweeteners); mineral water (cations, anions); wines and brandies (cations, organic acids, polyphenols, aldehydes, sulfite); beer (inorganic cations and anions, hop and bitter acids, sulfite, amino acids, organic acids, vitamins); milk (proteins, antibiotics); tea, coffee (caffeine, polyphenols); foodstuff (amino acids, synthetic dyes, organic acids, amines, proteins, melamine);
Agriculture: grain and grain products (amino acids), mixed fodders (cations, amino acids, amines, vitamins);
Chemical industry: technological monitoring, analysis of raw material;
Pharmaceutical industry: patent medicines analysis, technological monitoring, and enantiomers separation;
Biochemistry: analysis of proteins by capillary isoelectric focusing technique, pharmacokinetics studies;
Forensic studies: analysis of explosives and trace detection, drugs analysis, analysis of writing paper components.
Analytical Characteristics
Detectors
- CAPEL-105M is equipped with a spectrophotometric detector with a wavelength range of 190 - 400 nm.
Light source
Vials
- Standard Eppendorf-type 1.5 ml, minimal sample volume 100 µl.
Injection modes
- Sample injections by pressure, vacuum or electrokinetically.
- Controlled pressure profile of injection with pressure values from – 99 mbar to + 99 mbar.
- Electrokinetic injection at (+/-) 1 – 25 kV considering time for voltage ramping.
- Programmable injection time / pressure / voltage.
Analysis
- With a Voltage from -25 kV to +25 kV with a 1 kV step and an electronic polarity switch.
- Current from 0 to 200 µA.
Elforun software features
- Combination of instrument controlling, data acquisition and data processing;
- Real time electropherogram visualization;
- Electropherogram data processing with overlay mode and basic mathematical operations;
- Computation of electrophoresis system parameters;
- Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors;
- Extensive data processing algorithm customization;
- GLP and GALP compliance.
Equipment and Options
- Capillary electrophoresis system CAPEL-105M;
- Spare capillary cassettes;
- Elforun software package;
- Kits for analyses (by request); most of CE-kits of any manufacturers are compatible with Capel-105M.
Technical Specifications
Detector :
- Spectrophotometer 190-400 nm
Analysis :
- Constant voltage (+/-) 1 - 25 kV in 1 kV steps
- Automatic (electronic) polarity switch
- Current 0 - 200 µA
- Pressure (+/-) 1-99 mbar
- Programmable changing of wavelength, pressure and voltage during analysis
Injection :
- By voltage
- By pressure
- By vacuum
Sampler :
- Autosamplers for 10 inlet and 10 outlet vials
Rinsing :
With pressure ~1000 or 2000 mbar
Capillary :
- Length: 30-100 cm
- Internal diameter: 50, 75, 100 μm
Capillary thermostating :
- Liquid thermostating conducted at temperatures from 10 ºC below to 30ºC above ambient temperature (± 0.1 C)
Controlled parameters :
- Wavelength
- Pressure
- Temperature
- Voltage
Power requirements :
Power consumption :
Size :
Weight :
